Invitrogen

MERTK Monoclonal Antibody (DS5MMER), Alexa Fluor™ 700, eBioscience™

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MERTK Antibody (56-5751-80) in Flow

Mouse resident peritoneal exudate cells were stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and 0.5 µg of Rat IgG2a K Isotype Control Antibody, Alexa Fluor 700 (Product # 56-4321-80) (left) or 0.5 µg of MerTK Monoclonal Antibody, Alexa Fluor 700 (right). Total viable cells were used for analysis.

MERTK Antibody in Immunohistochemistry (IHC)

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Product Details

56-5751-80

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS5MMER

Immunogen

FC-tagged full protein expressed in insect cells

View immunogen

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784770

Product Specific Information

Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines. In addition MerTK may function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies. MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium. This process can be enhanced by stimulation with LPS. MerTK can be used to help discriminate macrophages from dendritic cells. MerTK is often expressed on malignant cells and may be implicated in immune evasion.

The DS5MMER antibody will work on paraformaldehyde-fixed cells.

Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS5MMER antibody has been tested by flow cytometric analysis of resident peritoneal macrophages. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

Target Information

MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Pop OT,Geng A,Flint E,Singanayagam A,Ercan C,Possamai L,Patel VC,Kuenzler P,Meier MA,Soysal S,Hruz P,Kollmar O,Tatham KC,Ward JK,Müllhaupt B,Weber A,Wendon J,Niess JH,Heim M,Semela D,Weston C,Antoniades CG,Terracciano LM,Triantafyllou E,Brenig RG,Bernsmeier C

56-5751-82 was used in Flow Cytometry to compare AXL expression in liver biopsies in cirrhosis (n = 22), chronic liver disease (n = 8), non-cirrhotic portal hypertension (n = 4), and healthy controls (n = 4).

Mon Jun 26 00:00:00 EDT 2023

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

Published figure using MERTK monoclonal antibody (Product # 56-5751-82) in Immunohistochemistry

Thu Dec 01 00:00:00 EST 2022

Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages.

Cell death & disease

Wanke F,Gutbier S,Rümmelin A,Steinberg M,Hughes LD,Koenen M,Komuczki J,Regan-Komito D,Wagage S,Hesselmann J,Thoma R,Brugger D,Christopeit T,Wang H,Point F,Hallet R,Ghosh S,Rothlin CV,Patsch C,Geering B

Published figure using MERTK monoclonal antibody (Product # 56-5751-82) in Flow Cytometry

Tue May 25 00:00:00 EDT 2021

Fgr kinase is required for proinflammatory macrophage activation during diet-induced obesity.

Nature metabolism

Acín-Pérez R,Iborra S,Martí-Mateos Y,Cook ECL,Conde-Garrosa R,Petcherski A,Muñoz MDM,Martínez de Mena R,Krishnan KC,Jiménez C,Bolaños JP,Laakso M,Lusis AJ,Shirihai OS,Sancho D,Enríquez JA

56-5751-82 was used in Flow cytometry/Cell sorting to investigate if ablation of Fgr tyrosine kinase impairs proinflammatory macrophage polarization while preventing high-fat diet (HFD)-induced obesity in mice.

Tue Sep 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: MGC133349; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase Mer; Receptor tyrosine kinase MerTK; sMER; sMERTK; soluble MER; soluble MERTK; Tyro 12; Tyrosine-protein kinase Mer

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Gene Aliases: Eyk; Mer; Mertk; nmf12; Nyk

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UniProt ID: (Mouse) Q60805

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Entrez Gene ID: (Mouse) 17289

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

natural killer cell differentiation protein phosphorylation phagocytosis spermatogenesis phosphorylation platelet activation secretion by cell substrate adhesion-dependent cell spreading apoptotic cell clearance protein kinase B signaling positive regulation of phagocytosis negative regulation of lymphocyte activation retina development in camera-type eye vagina development negative regulation of leukocyte apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MERTK Monoclonal Antibody (DS5MMER), Alexa Fluor™ 700, eBioscience™

MERTK Antibody (56-5751-80) in Flow

Product Details

56-5751-80

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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